Tuesday, April 2, 2013

Mechanism of mutant histone protein in childhood brain cancer revealed

Apr. 1, 2013 ? Most cancer treatments are blunt. In an attempt to eradicate tumors, oncologists often turn to radiation or chemotherapy, which can damage healthy tissue along with the cancerous growths. New research from C. David Allis' laboratory at Rockefeller University may bring scientists closer to designing cancer therapeutics that can target tumors with pinpoint accuracy.

Their findings, published in Science Express, follow a recent series of discoveries by several international genome sequencing consortiums that directly links a mutated histone protein to a rare brain stem cancer in children called DIPG. Collectively, these studies represented the first time scientists had linked a histone mutation to a disease, and piqued the interest of Peter Lewis, a research associate in Allis' Laboratory of Chromatin Biology and Epigenetics, who spearheaded these new studies.

Together with DNA, histones comprise the gene packaging material called chromatin. The mutation occurs on histone H3, and involves the remarkably specific substitution of one amino acid, lysine, for another, methionine, at a key position on the histone's tail, "silencing" the associated gene. Normally, gene silencing arises when an enzyme called a methyltransferase, containing a structural region called the SET domain, attaches a methyl chemical group to the lysine at position 27 in the H3 tail. This highly specific chemical reaction, called methylation, is disrupted by the replacement of the lysine with methionine, which could result in gene mis-regulation.

Lewis and his colleagues looked at human DIPG tumors that contained the lysine-to-methionine substitution and determined that mutated histone H3 comprised anywhere from 3.6 percent to 17.6 percent of total H3 in DIPG samples. They also found a global reduction in the levels of methylation of normal H3 histones when small amounts of the mutant H3 were added to normal human cells.

"I have often said, 'Every amino acid in histones matters,'" says Allis, who is the Joy and Jack Fishman Professor. "These studies underscore just how true that may be."

The researchers went on to demonstrate that the reduction in methylation of normal H3 histones results from interference with activity of a methyltransferase called PRC2 by the mutant histone. Methylation of normal H3 by PRC2 leads to repression of genes involved in cellular growth pathways. Without methylation, genes involved in these pathways likely become activated, promoting the growth of tumors in DIPG. Allis and Lewis received funding from the National Institutes of Health and the Starr Cancer Consortium. Key collaborators in this work included Oren Becher at Duke University Medical Center and Tom Muir at Princeton University and their colleagues.

"Our finding provides us with a useful tool for probing biological processes," says Lewis. "This also tells us how to inhibit enzymes, which could lead to the development of pharmaceuticals that mimic the action of these mutants."

"We now have a model for the promotion of brain stem cancers through aberrant epigenetic silencing through the inhibition of PRC2 by a mutant histone," says Allis. "We have uncovered a potentially useful mechanism to exclusively inhibit individual SET-domain methyltransferases, and conceivably other chromatin-modifying enzymes, implicated in a variety of malignancies."

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The above story is reprinted from materials provided by Rockefeller University.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. P. W. Lewis, M. M. Muller, M. S. Koletsky, F. Cordero, S. Lin, L. A. Banaszynski, B. A. Garcia, T. W. Muir, O. J. Becher, C. D. Allis. Inhibition of PRC2 Activity by a Gain-of-Function H3 Mutation Found in Pediatric Glioblastoma. Science, 2013; DOI: 10.1126/science.1232245

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/~3/40YXt_m5Bp8/130401132054.htm

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AP IMPACT: Cartels dispatch agents deep inside US

In this Feb. 14, 2013 photo, Art Bilek, executive vice president of the Chicago Crime Commission, left, announces that Joaquin ``El Chapo'' Guzman, a drug kingpin in Mexico, has been named Chicago's Public Enemy No. 1, during a news conference in Chicago. Looking on is Jack Riley, right, head of the Drug Enforcement Administration in Chicago and Peter Bensinger, former Administrator of the United States DEA. Ruthless drug cartels have long been the nation?s No. 1 supplier of illegal drugs, but in the past, their operatives rarely ventured beyond the border. A wide-ranging Associated Press review of federal court cases and government drug-enforcement data, plus interviews with many top law enforcement officials, indicate the groups have begun deploying agents from their inner circles to the U.S. (AP Photo/M. Spencer Green)

In this Feb. 14, 2013 photo, Art Bilek, executive vice president of the Chicago Crime Commission, left, announces that Joaquin ``El Chapo'' Guzman, a drug kingpin in Mexico, has been named Chicago's Public Enemy No. 1, during a news conference in Chicago. Looking on is Jack Riley, right, head of the Drug Enforcement Administration in Chicago and Peter Bensinger, former Administrator of the United States DEA. Ruthless drug cartels have long been the nation?s No. 1 supplier of illegal drugs, but in the past, their operatives rarely ventured beyond the border. A wide-ranging Associated Press review of federal court cases and government drug-enforcement data, plus interviews with many top law enforcement officials, indicate the groups have begun deploying agents from their inner circles to the U.S. (AP Photo/M. Spencer Green)

In this Dec. 11, 2012 file photo, Jack Riley, head of the Drug Enforcement Administration in Chicago, points out local Mexican drug cartel problem areas on a map in the new interagency Strike Force office in Chicago. Looking on is DEA agent Vince Balbo. The ruthless syndicates have long been the nation?s No. 1 supplier of illegal drugs, but in the past, their operatives rarely ventured beyond the border. A wide-ranging Associated Press review of federal court cases and government drug-enforcement data, plus interviews with many top law enforcement officials, indicate the groups have begun deploying agents from their inner circles to the U.S. (AP Photo/M. Spencer Green, File)

This 2009 photo provided by the Gwinnett County Sheriff's Department in Lawrenceville, Ga., shows reputed cartel operative Socorro Hernandez-Rodriguez after his arrest in a suburb of Atlanta. Hernandez-Rodriguez was later convicted of sweeping drug trafficking charges. Prosecutors said he was a high-ranking figure in the La Familia cartel, sent to the U.S. to run a drug cell. His defense lawyers denied he was a major figure in the cartel. (AP Photo/Courtesy of the Gwinnett County Sheriff?s Department)

This photo dated in 2007 from federal court documents provided by attorneys for Jose Gonzales-Zavala shows Gonzales-Zavala with two of his children allegedly taken in Mexico. Prosecutors say Gonzales-Zavala was a member of the La Familia cartel, based in southwestern Mexico, and dispatched to the Chicago area to oversee one of the cartel's lucrative trafficking cells. His defense team entered the photograph into evidence during the sentence stage of his case in arguing for leniency. In 2011, he was sentenced to 40 years in prison by a federal judge in Chicago. (AP Photo/Attorneys for Jose Gonzales-Zavala)

FILE - In this Oct. 22, 2009 file photo, weapons and drugs seized in special joint operation conducted with the Drug Enforecement Administration against the La Familia drug cartel based out of Michoacan, Mexico and operating in San Bernardino and surrounding counties, are on display at a news conference at sheriff's headquarters in San Bernardino, Calif. Drug cartels have long been the nation?s No. 1 supplier of illegal drugs, but in the past, their operatives rarely ventured beyond the border. A wide-ranging Associated Press review of federal court cases and government drug-enforcement data, plus interviews with many top law enforcement officials, indicate the groups have begun deploying agents from their inner circles to the U.S. (AP Photo/Reed Saxon, File)

(AP) ? Mexican drug cartels whose operatives once rarely ventured beyond the U.S. border are dispatching some of their most trusted agents to live and work deep inside the United States ? an emboldened presence that experts believe is meant to tighten their grip on the world's most lucrative narcotics market and maximize profits.

If left unchecked, authorities say, the cartels' move into the American interior could render the syndicates harder than ever to dislodge and pave the way for them to expand into other criminal enterprises such as prostitution, kidnapping-and-extortion rackets and money laundering.

Cartel activity in the U.S. is certainly not new. Starting in the 1990s, the ruthless syndicates became the nation's No. 1 supplier of illegal drugs, using unaffiliated middlemen to smuggle cocaine, marijuana and heroin beyond the border or even to grow pot here.

But a wide-ranging Associated Press review of federal court cases and government drug-enforcement data, plus interviews with many top law enforcement officials, indicate the groups have begun deploying agents from their inner circles to the U.S. Cartel operatives are suspected of running drug-distribution networks in at least nine non-border states, often in middle-class suburbs in the Midwest, South and Northeast.

"It's probably the most serious threat the United States has faced from organized crime," said Jack Riley, head of the Drug Enforcement Administration's Chicago office.

The cartel threat looms so large that one of Mexico's most notorious drug kingpins ? a man who has never set foot in Chicago ? was recently named the city's Public Enemy No. 1, the same notorious label once assigned to Al Capone.

The Chicago Crime Commission, a non-government agency that tracks crime trends in the region, said it considers Joaquin "El Chapo" Guzman even more menacing than Capone because Guzman leads the deadly Sinaloa cartel, which supplies most of the narcotics sold in Chicago and in many cities across the U.S.

Years ago, Mexico faced the same problem ? of then-nascent cartels expanding their power ? "and didn't nip the problem in the bud," said Jack Killorin, head of an anti-trafficking program in Atlanta for the Office of National Drug Control Policy. "And see where they are now."

Riley sounds a similar alarm: "People think, 'The border's 1,700 miles away. This isn't our problem.' Well, it is. These days, we operate as if Chicago is on the border."

Border states from Texas to California have long grappled with a cartel presence. But cases involving cartel members have now emerged in the suburbs of Chicago and Atlanta, as well as Columbus, Ohio, Louisville, Ky., and rural North Carolina. Suspects have also surfaced in Indiana, Michigan, Minnesota and Pennsylvania.

Mexican drug cartels "are taking over our neighborhoods," Pennsylvania Attorney General Kathleen Kane warned a legislative committee in February. State Police Commissioner Frank Noonan disputed her claim, saying cartels are primarily drug suppliers, not the ones trafficking drugs on the ground.

For years, cartels were more inclined to make deals in Mexico with American traffickers, who would then handle transportation to and distribution within major cities, said Art Bilek, a former organized crime investigator who is now executive vice president of the crime commission.

As their organizations grew more sophisticated, the cartels began scheming to keep more profits for themselves. So leaders sought to cut out middlemen and assume more direct control, pushing aside American traffickers, he said.

Beginning two or three years ago, authorities noticed that cartels were putting "deputies on the ground here," Bilek said. "Chicago became such a massive market ... it was critical that they had firm control."

To help fight the syndicates, Chicago recently opened a first-of-its-kind facility at a secret location where 70 federal agents work side-by-side with police and prosecutors. Their primary focus is the point of contact between suburban-based cartel operatives and city street gangs who act as retail salesmen. That is when both sides are most vulnerable to detection, when they are most likely to meet in the open or use cellphones that can be wiretapped.

Others are skeptical about claims cartels are expanding their presence, saying law-enforcement agencies are prone to exaggerating threats to justify bigger budgets.

David Shirk, of the University of San Diego's Trans-Border Institute, said there is a dearth of reliable intelligence that cartels are dispatching operatives from Mexico on a large scale.

"We know astonishingly little about the structure and dynamics of cartels north of the border," Shirk said. "We need to be very cautious about the assumptions we make."

Statistics from the DEA suggest a heightened cartel presence in more U.S. cities. In 2008, around 230 American communities reported some level of cartel presence. That number climbed to more than 1,200 in 2011, the most recent year for which information is available, though the increase is partly due to better reporting.

Federal agents and local police say they have become more adept at identifying cartel members or operatives using wiretapped conversations, informants or confessions. Hundreds of court documents reviewed by the AP appear to support those statements.

"This is the first time we've been seeing it ? cartels who have their operatives actually sent here," said Richard Pearson, a lieutenant with the Louisville Metropolitan Police Department, which arrested four alleged operatives of the Zetas cartel in November in the suburb of Okolona.

People who live on the tree-lined street where authorities seized more than 2,400 pounds of marijuana and more than $1 million in cash were shocked to learn their low-key neighbors were accused of working for one of Mexico's most violent drug syndicates, Pearson said.

One of the best documented cases is Jose Gonzalez-Zavala, who was dispatched to the U.S. by the La Familia cartel, according to court filings.

In 2008, the former taxi driver and father of five moved into a spacious home at 1416 Brookfield Drive in a middle-class neighborhood of Joliet, southwest of Chicago. From there, court papers indicate, he oversaw wholesale shipments of cocaine in Illinois, Wisconsin and Indiana.

Wiretap transcripts reveal he called an unidentified cartel boss in Mexico almost every day, displaying the deference any midlevel executive might show to someone higher up the corporate ladder. Once he stammered as he explained that one customer would not pay a debt until after a trip.

"No," snaps the boss. "What we need is for him to pay."

The same cartel assigned Jorge Guadalupe Ayala-German to guard a Chicago-area stash house for $300 a week, plus a promised $35,000 lump-sum payment once he returned to Mexico after a year or two, according to court documents.

Ayala-German brought his wife and child to help give the house the appearance of an ordinary family residence. But he was arrested before he could return home and pleaded guilty to multiple trafficking charges. He will be sentenced later this year.

Socorro Hernandez-Rodriguez was convicted in 2011 of heading a massive drug operation in suburban Atlanta's Gwinnett County. The chief prosecutor said he and his associates were high-ranking figures in the La Familia cartel ? an allegation defense lawyers denied.

And at the end of February outside Columbus, Ohio, authorities arrested 34-year-old Isaac Eli Perez Neri, who allegedly told investigators he was a debt collector for the Sinaloa cartel.

An Atlanta attorney who has represented reputed cartel members says authorities sometimes overstate the threat such men pose.

"Often, you have a kid whose first time leaving Mexico is sleeping on a mattress at a stash house playing Game Boy, eating Burger King, just checking drugs or money in and out," said Bruce Harvey. "Then he's arrested and gets a gargantuan sentence. It's sad."

Typically, cartel operatives are not U.S. citizens and make no attempt to acquire visas, choosing instead to sneak across the border. They are so accustomed to slipping back and forth between the two countries that they regularly return home for family weddings and holidays, Riley said.

Because cartels accumulate houses full of cash, they run the constant risk associates will skim off the top. That points to the main reason cartels prefer their own people: Trust is hard to come by in their cutthroat world. There's also a fear factor. Cartels can exert more control on their operatives than on middlemen, often by threatening to torture or kill loved ones back home.

Danny Porter, chief prosecutor in Gwinnett County, Ga., said he has tried to entice dozens of suspected cartel members to cooperate with American authorities. Nearly all declined. Some laughed in his face.

"They say, 'We are more scared of them (the cartels) than we are of you. We talk and they'll boil our family in acid,'" Porter said. "Their families are essentially hostages."

Citing the safety of his own family, Gonzalez-Zavala declined to cooperate with authorities in exchange for years being shaved off his 40-year sentence.

In other cases, cartel brass send their own family members to the U.S.

"They're sometimes married or related to people in the cartels," Porter said. "They don't hire casual labor." So meticulous have cartels become that some even have operatives fill out job applications before being dispatched to the U.S., Riley added.

In Mexico, the cartels are known for a staggering number of killings ? more than 50,000, according to one tally. Beheadings are sometimes a signature.

So far, cartels don't appear to be directly responsible for large numbers of slayings in the United States, though the Texas Department of Public Safety reported 22 killings and five kidnappings in Texas at the hands of Mexican cartels from 2010 through mid- 2011.

Still, police worry that increased cartel activity could fuel heightened violence.

In Chicago, the police commander who oversees narcotics investigations, James O'Grady, said street-gang disputes over turf account for most of the city's uptick in murders last year, when slayings topped 500 for the first time since 2008. Although the cartels aren't dictating the territorial wars, they are the source of drugs.

Riley's assessment is stark: He argues that the cartels should be seen as an underlying cause of Chicago's disturbingly high murder rate.

"They are the puppeteers," he said. "Maybe the shooter didn't know and maybe the victim didn't know that. But if you follow it down the line, the cartels are ultimately responsible."

___

Follow Michael Tarm at www.twitter.com/mtarm .

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/3d281c11a96b4ad082fe88aa0db04305/Article_2013-04-01-Cartels-Coming%20to%20America/id-c5632c7893744bd1931ed864dc864b32

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Monday, April 1, 2013

Chinese Rescuers Find More Bodies in Tibet Landslide (Voice Of America)

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Insert Coin: Dash charts your car data live, with gauges and a dashcam (video)

In Insert Coin, we look at an exciting new tech project that requires funding before it can hit production. If you'd like to pitch a project, please send us a tip with "Insert Coin" as the subject line.

Insert Coin Drive with Dash charts car data live, with gauges and dashcams video

We've seen a few stabs at smartphone-enhanced car diagnostics as of late, but many good solutions like Automatic Link and Delphi's Vehicle Diagnostics are primarily useful after you've parked. The upcoming Dash OBD-II adapter is certainly up to that side of the job, telling a Bluetooth-connected iOS device (and eventually, Android) about your car's problems and estimating fuel costs based on the gas tank's levels. Where it stands out is its usefulness while on the road: the custom app offers custom live gauges, including a Green-Meter for ideal fuel economy that you won't usually find in a real instrument cluster. There's even a dashcam mode that overlays travel details on captured video, whether it's to support insurance claims or just to immortalize a drive through the back country.

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Source: Kickstarter

Source: http://www.engadget.com/2013/03/31/insert-coin-dash-charts-your-car-data-live-with-gauges-and-dashcam/

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NKorea calls nukes country's 'life' at big meeting

South Korean Army soldiers patrol along a barbed-wire fence near the border village of Panmunjom in Paju, South Korea, Sunday, March 31, 2013. North Korea warned South Korea on Saturday that the Korean Peninsula had entered "a state of war" and threatened to shut down a border factory complex that's the last major symbol of inter-Korean cooperation. (AP Photo/Ahn Young-joon)

South Korean Army soldiers patrol along a barbed-wire fence near the border village of Panmunjom in Paju, South Korea, Sunday, March 31, 2013. North Korea warned South Korea on Saturday that the Korean Peninsula had entered "a state of war" and threatened to shut down a border factory complex that's the last major symbol of inter-Korean cooperation. (AP Photo/Ahn Young-joon)

South Korean Army soldiers patrol along a barbed-wire fence near the border village of Panmunjom in Paju, South Korea, Sunday, March 31, 2013. North Korea warned South Korea on Saturday that the Korean Peninsula had entered "a state of war" and threatened to shut down a border factory complex that's the last major symbol of inter-Korean cooperation. (AP Photo/Ahn Young-joon)

South Korean Army soldiers patrol along a barbed-wire fence near the border village of Panmunjom in Paju, South Korea, Sunday, March 31, 2013. North Korea warned South Korea on Saturday that the Korean Peninsula had entered "a state of war" and threatened to shut down a border factory complex that's the last major symbol of inter-Korean cooperation. (AP Photo/Ahn Young-joon)

(AP) ? A top North Korean decision-making body issued a pointed warning Sunday, saying that nuclear weapons are "the nation's life" and will not be traded even for "billions of dollars."

The comments came in a statement released after North Korean leader Kim Jong Un presided over the plenary meeting of the central committee of the ruling Workers' Party. The meeting, which set a "new strategic line" calling for building both a stronger economy and nuclear arsenal, comes amid a series of near-daily threats from Pyongyang in recent weeks, including a vow to launch nuclear strikes on the United States and a warning Saturday that the Korean Peninsula was in a "state of war."

Pyongyang is angry over annual U.S.-South Korean military drills and a new round of U.N. sanctions that followed its Feb. 12 nuclear test, the country's third. Analysts see a full-scale North Korean attack as unlikely and say the threats are more likely efforts to provoke softer policies toward Pyongyang from a new government in Seoul, to win diplomatic talks with Washington that could get the North more aid, and to solidify the young North Korean leader's image and military credentials at home.

North Korea made reference to those outside views in the statement it released through the official Korean Central News Agency following the plenary meeting.

North Korea's nuclear weapons are a "treasure" not to be traded for "billions of dollars," the statement said. They "are neither a political bargaining chip nor a thing for economic dealings to be presented to the place of dialogue or be put on the table of negotiations aimed at forcing (Pyongyang) to disarm itself," it said.

North Korea's "nuclear armed forces represent the nation's life, which can never be abandoned as long as the imperialists and nuclear threats exist on earth," the statement said.

North Korea has called the U.S. nuclear arsenal a threat to its existence since the 1950-53 Korean War, which ended in a truce, not a peace treaty, leaving the peninsula still technically at war. Pyongyang justifies its own nuclear pursuit in large part on that perceived U.S. threat.

While analysts call North Korea's threats largely brinkmanship, there is some fear that a localized skirmish might escalate. Seoul has vowed to respond harshly should North Korea provoke its military. Naval skirmishes in disputed Yellow Sea waters off the Korean coast have led to bloody battles several times over the years. Attacks blamed on Pyongyang in 2010 killed 50 South Koreans.

The plenary statement also called for strengthening the moribund economy, which Kim has put an emphasis on in his public statements since taking power after the death of his father, Kim Jong Il, in late 2011. The United Nations says two-thirds of the country's 24 million people face regular food shortages.

The statement called for diversified foreign trade and investment, and a focus on agriculture, light industry and a "self-reliant nuclear power industry," including a light water reactor. There was also a call for "the development of space science and technology," including more satellite launches. North Korea put a satellite into orbit on a long-range rocket in December. The United Nations called the launch a cover for a banned test of ballistic missile technology and increased sanctions on the North.

The central committee is a top decision-making body of the North's ruling Workers' Party. The committee is tasked with organizing and guiding the party's major projects, and its plenary meeting is usually convened once a year, according to Seoul's Unification Ministry. South Korean media said the last plenary session was held in 2010 and that this was the first time Kim Jong Un had presided over the meeting.

The White House says the United States is taking North Korea's threats seriously, but has also noted Pyongyang's history of "bellicose rhetoric."

On Thursday, U.S. military officials revealed that two B-2 stealth bombers dropped dummy munitions on an uninhabited South Korean island as part of annual defense drills that Pyongyang sees as rehearsals for invasion. Hours later, Kim ordered his generals to put rockets on standby and threatened to strike American targets if provoked.

___

Associated Press writer Hyung-jin Kim contributed to this report. Follow Foster Klug at www.twitter.com/APKlug.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/3d281c11a96b4ad082fe88aa0db04305/Article_2013-03-31-Koreas-Tension/id-606cf31256544346ab62310d9e16346e

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Alaska Troopers helicopter crashes during rescue

ANCHORAGE, Alaska (AP) ? An Alaska State Trooper helicopter carrying two troopers and a rescued snowmobiler crashed Saturday night in the south-central part of the state, and no survivors have been found, an agency spokeswoman said.

Trooper spokeswoman Megan Peters said the crash site was spotted Sunday, but she could not immediately confirm that the three on board were killed.

Wreckage of the helicopter burned, but Peters said it was not known how the fire started or how long it lasted.

The trooper helicopter was on a mission to pick up a snowmobiler stranded near Larson Lake 7 miles east of Talkeetna in the Matanuska-Susitna Borough.

The helicopter picked up a trooper and began a search, spotting the snowmobile and landing just before 10 p.m. It took off with the snowmobiler and was supposed to meet medics near Talkeetna but did not arrive.

The National Weather Service said the temperature in Talkeetna Sunday night was 34 with light rain that turned into snow at about 11 p.m., but that visibility was 10 miles.

Peters said it's not known if weather was a factor in the crash. The National Transportation Safety Board will investigate.

Alaska Air National Guard helicopter spokeswoman Kalei Rupp said fog in Anchorage and mandatory crew rest kept an agency helicopter from launching until 5 a.m.

A guard helicopter spotted the wreckage at 9:20 a.m. Sunday and landed.

Talkeetna is a community of nearly 900 about 80 miles north of Anchorage. It's the primary departure point for air taxis taking climbers to Mount McKinley.

The crash site was near the south end of Larson Lake.

The Alaska State Troopers have had at least one officer die in a helicopter crash during a search and rescue mission.

Trooper John David Stimson was on a chartered helicopter in 1983 that was attempting to reach a downed pilot in a blizzard. The chartered helicopter crashed near Cordova.

Stimson survived and assisted pilot Gary Wiltrout, who had suffered a broken back. However, when rescuers reached the crash site after 24 hours, the 11-year trooper had died of exposure. Wiltrout survived.

Source: http://news.yahoo.com/alaska-troopers-helicopter-crashes-during-rescue-001713287.html

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Pope presides over trimmed Easter Vigil service

VATICAN CITY (AP) ? Pope Francis celebrated a trimmed back Easter Vigil service Saturday after having reached out to Muslims and women during a Holy Week in which he began to put his mark on the Catholic Church.

Francis processed into a darkened and silent St. Peter's Basilica at the start of the service, in which the faithful recall the period between Christ's crucifixion on Good Friday and resurrection on Easter Sunday.

One of the most dramatic moments of the Easter Vigil service that usually follows ? when the pope would share the light of his candle with others until the entire basilica twinkled ? was shortened this year as were some of the Old Testament readings.

The Vatican has said these provisions were in keeping with Francis' aim to not have his Masses go on too long. The Easter Vigil service under Benedict XVI would typically run nearly three hours. The new pope has made clear he prefers his Masses short and to the point: he was even caught checking his watch during his March 19 installation ceremony. Saturday was no different: The vigil ended just shy of 2.5 hours.

A trimmed-back vigil ? and one that started earlier than usual ? was just one of the novelties of this Holy Week under an Argentine Jesuit pope who just two weeks ago stunned the world by emerging from the loggia of St. Peter's Basilica after his election with a simple "Brothers and sisters, good evening."

He riled traditionalists but endeared himself to women and liberals by washing and kissing the feet of two young girls during a Holy Thursday Mass at a juvenile detention center in Rome, when the rite usually calls for only men to participate. A day later, Francis reached out with friendship to "Muslim brothers and sisters" during a Good Friday procession dedicated to the suffering of Christians from terrorism, war and religious fanaticism in the Middle East.

In his homily Saturday, Francis kept his message simple and tied to the liturgical readings, recalling how Jesus' disciples found his tomb empty a day after his death and were surprised and confused.

"Our daily problems and worries can wrap us up in ourselves, in sadness and bitterness, and that is where death is," he said. "Let the risen Jesus enter your life, welcome him as a friend, with trust: he is life!"

He later baptized four men, part of the Easter Vigil ritual.

Just a few hours after the vigil ends, Francis on Sunday will celebrate Easter Mass and deliver his "Urbi et Orbi" speech, Latin for "To the city and the world." Usually the pope also issues Easter greetings in dozens of languages.

In his two weeks as pope, Francis' discomfort with speaking in any language other than Italian has become apparent. He has even shied away from speaking Spanish when the occasion would call for it, though the Vatican has said he has done so to avoid discriminating against other languages by favoring his native tongue.

Italian is the lingua franca of the Vatican and Francis has emphasized his role as bishop of Rome over that of pope of the universal church, making his use of Italian logical.

It's not clear how Francis will handle the multilingual greetings Sunday.

Typically, after the busy Easter week ceremonies, the pope would go to the papal retreat at Castel Gandolfo for a few days of vacation. Francis can't do that since the previous pope, Benedict XVI, is currently living there in retirement.

The Vatican has said Francis would stay put in the Vatican.

___

Follow Nicole Winfield at www.twitter.com/nwinfield

Source: http://news.yahoo.com/pope-presides-over-trimmed-easter-vigil-200621332.html

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